Reproduction Abstracts

Introduction: The type II bacterial clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated protein (Cas) have been proven to be an effective gene targeting system. Genome editing of non-rodent mammalian species is a promising strategy for generation of animal models for human diseases. Mashiko et al. reported a high efficacy of direct injection of a plasmid DNA, encoding humanized Cas9 and sgRNA (single-guide RNA) in mice. Here we show successf...